from cellpose import models, plot from cellpose.io import imread, save_masks from PIL import Image import numpy as np import os, datetime import time class Cprun: @classmethod def run_test(cls): model = models.CellposeModel(gpu=True) files = ['test_tif/img.png'] imgs = [imread(f) for f in files] masks, flows, styles = model.eval( imgs, flow_threshold=0.4, cellprob_threshold=0.0 ) for i, m in enumerate(masks): print( f"[{i}] mask max={int(getattr(m, 'max', lambda: 0)()) if hasattr(m, 'max') else int(np.max(m))}, unique={np.unique(m)[:5]} ..." ) ts = datetime.datetime.now().strftime("%Y-%m-%d-%H-%M-%S") + f"-{int(time.time()*1000)%1000:03d}" outdir = os.path.join(os.path.dirname(__file__), "test_output", ts) os.makedirs(outdir, exist_ok=True) # 自动创建目录 for img, mask, flow, name in zip(imgs, masks, flows, files): base = os.path.join(outdir, os.path.splitext(os.path.basename(name))[0]) #使用内置绘图生成蒙版 out = base + "_output" save_masks(imgs, mask, flow, out, tif=True) # 用 plot 生成彩色叠加图(不依赖 skimage) rgb = plot.image_to_rgb(img, channels=[0, 0]) # 原图转 RGB over = plot.mask_overlay(rgb, masks=mask, colors=None) # 叠加彩色实例 Image.fromarray(over).save(base + "_overlay.png") @classmethod async def run(cls, images: list[str] | str | None = None, time: str | None = None, model: str = "cpsam", diameter: float | None = None, flow_threshold: float | float = 0.4, cellprob_threshold: float | float = 0.0, ): if time is None: return [False, "No time received"] if images is None: return [False, "No images received"] message = [f"Using {model} model"] model = models.CellposeModel(gpu=True, model_type=model) files = images imgs = [imread(f) for f in files] masks, flows, styles = model.eval( imgs, flow_threshold=flow_threshold, cellprob_threshold=cellprob_threshold, diameter=diameter ) ts = time outdir = os.path.join(os.path.dirname(__file__), "output", ts) os.makedirs(outdir, exist_ok=True) # 自动创建目录 for img, mask, flow, name in zip(imgs, masks, flows, files): base = os.path.join(outdir, os.path.splitext(os.path.basename(name))[0]) # 使用内置绘图生成蒙版 out = base + "_output" save_masks(imgs, mask, flow, out, tif=True) # 用 plot 生成彩色叠加图(不依赖 skimage) rgb = plot.image_to_rgb(img, channels=[0, 0]) # 原图转 RGB over = plot.mask_overlay(rgb, masks=mask, colors=None) # 叠加彩色实例 Image.fromarray(over).save(base + "_overlay.png") message.append(f"Output saved to: {outdir}") message.append(outdir) return [True, message]